SiteFinding-PCR: a simple and efficient PCR method for chromosome walking

In this paper, we present a novel PCR method, termed SiteFinding-PCR, for gene or chromosome walking. The PCR was primed by a SiteFinder at a low temperature, and then the target molecules were amplified exponentially with gene-specific and SiteFinder primers, and screened out by another gene-specific primer and a vector primer. However, non-target molecules could not be amplified exponentially owing to the suppression effect of stem–loop structure and could not be screened out. This simple method proved to be efficient, reliable, inexpensive and time-saving, and may be suitable for the molecules for which gene-specific primers are available. More importantly, large DNA fragments can be obtained easily using this method. To demonstrate the feasibility and efficiency of SiteFinding-PCR, we employed this method to do chromosome walking and obtained 16 positive results from 17 samples

Multi - mechanism coalescence design and matrix expression of logic action sequences of the over-turn nursing robot Part II: Gesture-state in sets and matrix

As is expressed in Part I, Functions and coalescence design of the over-turn nursing robot, the performance and requirements have been put forward with systematic design of several mechanisms. Here, in order to control and function well the over-turn nursing robot, the three-dimensional and five-dimensional Euclidean space with the real number were adopted in terms of sets for gesture of the bedridden person and the corresponding state of the robot, respectively. The matrix method was employed to define and describe the gestures-robot performance and its transition path. The gesture-state sequence matrix not only accurately and clearly expressed the gesture series, state sequence and their corresponding relations, but also laid a theoretical and technical foundation for the path planning from the current gesture to the target one. The control and operation of 7 states and 5 gestures were done to realize the automation and intelligent over-turning safely, comfortably and conveniently

Multi - mechanism coalescence design and matrix expression of logic action sequences of the over-turn nursing robot Part I: Functions and coalescence design

In order to effectively solve the problem in over-turn of a bedridden person with the assistance of external force, a double bed face- three embedded leave over-turn nursing robot with the flexible compensation was put forward, with the abstraction of the bedridden person as an organism. This robot, on the basis of concept gesture of the person in bed and the state of the robot supporting and proving the gesture with the actions and combination of the two bed faces, held the complete function of over-turn nursing with 7 states corresponding to 5 gestures of the bedridden person obeying the fundamental requirements of safety, rapidity, and comport. The design method of "PS-MM-KD" was proposed for multi-mechanism coalescent system with related specific tasks induced from the original problems with Systems Engineering. Mechanics and Mechanisms, then applied in the concrete sub-system design followed by analysis and verification of both the scheme and the sub-systems in the design, using the Kinematics and Dynamics, implementing the gears, chain wheel, slewing mechanism, screw nut and mortise and tenon joint type clutch mechanism design successfully. Based on those above, a "two-bed face/three-leaf embedded flexible compensation nursing robot" was designed adopting to all ages, people of various kinds of body geometry. PLC, sensor and logic algorithm were used to carry out the control and operation of 7 state-5 posture sequences for realization of the automation and intelligent over-turning in safety, comfort, and convenience

Online synchronous inspection and system optimization of flexible food packaging bags by using machine vision and sensing technique

Flexible food packaging in the market is increasingly favored, and its quality is essential and indispensable for safety and convenience.  However, quality inspection still stays in the manual stage, or partially manual inspection remains, in production, leading low efficiency, lack and even false inspection, hardly meeting the requirements of the modern output.  This paper proposes and optimizes the design of an automatic detection system with intelligence for flexible food packaging bag, which can effectively be adopted to check the quality of packaging trademark patterns, fillers, and sealing quality.  The inspection system runs with two-stage structure, machine vision, pressure sensing and synchronization to improve efficiency and ensure the normal production beat. Simplex Method is adopted to determine the best synchronous speeds online to achieve the best expectation. Comparison has been made between the manual inspection and our automatic operation, the sample of 10000 was statistically analyzed and results have shown that two workers were saved and the correctness rate of inspection raised up to 999.8‰

The LSD1-Interacting Protein GILP Is a LITAF Domain Protein That Negatively Regulates Hypersensitive Cell Death in Arabidopsis

Hypersensitive cell death, a form of avirulent pathogen-induced programmed cell death (PCD), is one of the most efficient plant innate immunity. However, its regulatory mechanism is poorly understood. AtLSD1 is an important negative regulator of PCD and only two proteins, AtbZIP10 and AtMC1, have been reported to interact with AtLSD1.To identify a novel regulator of hypersensitive cell death, we investigate the possible role of plant LITAF domain protein GILP in hypersensitive cell death. Subcellular localization analysis showed that AtGILP is localized in the plasma membrane and its plasma membrane localization is dependent on its LITAF domain. Yeast two-hybrid and pull-down assays demonstrated that AtGILP interacts with AtLSD1. Pull-down assays showed that both the N-terminal and the C-terminal domains of AtGILP are sufficient for interactions with AtLSD1 and that the N-terminal domain of AtLSD1 is involved in the interaction with AtGILP. Real-time PCR analysis showed that AtGILP expression is up-regulated by the avirulent pathogen Pseudomonas syringae pv. tomato DC3000 avrRpt2 (Pst avrRpt2) and fumonisin B1 (FB1) that trigger PCD. Compared with wild-type plants, transgenic plants overexpressing AtGILP exhibited significantly less cell death when inoculated with Pst avrRpt2, indicating that AtGILP negatively regulates hypersensitive cell death.These results suggest that the LITAF domain protein AtGILP localizes in the plasma membrane, interacts with AtLSD1, and is involved in negatively regulating PCD. We propose that AtGILP functions as a membrane anchor, bringing other regulators of PCD, such as AtLSD1, to the plasma membrane. Human LITAF domain protein may be involved in the regulation of PCD, suggesting the evolutionarily conserved function of LITAF domain proteins in the regulation of PCD

An error-resilient and tunable distributed indexing scheme for wireless data broadcast

Abstract—Access efficiency and energy conservation are two critical performance concerns in a wireless data broadcast system. We propose in this paper a novel parameterized index called the exponential index that has a linear yet distributed structure for wireless data broadcast. Based on two tuning knobs, index base and chunk size, the exponential index can be tuned to optimize the access latency with the tuning time bounded by a given limit, and vice versa. The client access algorithm for the exponential index under unreliable broadcast is described. A performance analysis of the exponential index is provided. Extensive ns-2-based simulation experiments are conducted to evaluate the performance under various link error probabilities. Simulation results show that the exponential index substantially outperforms the state-of-the-art indexes. In particular, it is more resilient to link errors and achieves more performance advantages from index caching. The results also demonstrate its great flexibility in trading access latency with tuning time. Index Terms—Index structure, data broadcast, energy conservation, mobile computing.

Thermal energy storage using calcium chloride hexahydrate

The solid-liquid phase change material named as PCM-Ca with 49.1% in mass water, 49.7% calcium chloride, and 1.2% strontium chloride was discovered and outlined in this paper. Differential scanning calorimetry, simultaneous thermal analyzer, thermal conductivity analyzer, temperature recorder, and digital vibrating-tube densimeter were used to characterize the thermal properties of PCM-Ca. The results indicated that PCM-Ca has the phase change temperature at 28.7°C, the latent heats in the process of fusing and crystallizing are 193.4 and 193.0 kJ/kg, the thermal conductivity is 1.2801 W/mK at the melting point, and the density is 1.48 ⋅103 kg/m3 at room temperature. Thirty times repeated thermal cycling tests show excellent thermal stability in terms of latent heat and nucleation temperature, and the maximum deviations of the latent heat and the phase change temperature of PCM-Ca are 0.2% and 2.7%, respectively

Overview of Multi-Robot Collaborative SLAM from the Perspective of Data Fusion

In the face of large-scale environmental mapping requirements, through the use of lightweight and inexpensive robot groups to perceive the environment, the multi-robot cooperative (V)SLAM scheme can resolve the individual cost, global error accumulation, computational load, and risk concentration problems faced by single-robot SLAM schemes. Such schemes are robust and stable, form a current research hotspot, and relevant algorithms are being updated rapidly. In order to enable the reader to understand the development of this field rapidly and fully, this paper provides a comprehensive review. First, the development history of multi-robot collaborative SLAM is reviewed. Second, the fusion algorithms and architectures are detailed. Third, from the perspective of machine learning classification, the existing algorithms in this field are discussed, including the latest updates. All of this will make it easier for readers to discover problems that need to be studied further. Finally, future research prospects are listed